maxima first strand cdna synthesis kit (Thermo Fisher)
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Maxima First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxima+first+strand+cdna+synthesis+kit/revertaid+first+strand+cdna+synthesis+kit/pmc12221739-160-16-22
Average 90 stars, based on 1 article reviews
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1) Product Images from "mRNA-based in vivo induction of ETV2 to restore blood flow in a preclinical mouse hindlimb ischemia model"
Article Title: mRNA-based in vivo induction of ETV2 to restore blood flow in a preclinical mouse hindlimb ischemia model
Journal: Molecular Therapy. Nucleic Acids
doi: 10.1016/j.omtn.2025.102592
Figure Legend Snippet: Synthetic mRNA encoding ETV2 is efficiently translated into functional protein in vitro (A) EGFP or ETV2 protein expression at 15 h after mRNA transfection with MessengerMAX. Representative blight filed image and fluorescence image. Scale bars, 75 μm. TF, transfection reagent. (B) After mRNA transfection with MessengerMAX, the cells were collected at 24, 48, or 72 h time point. Total RNA extraction, cDNA synthesis, and qPCR analysis by ΔΔCT method were conducted. For each condition, n = 4; mean ± SD. ∗∗ p < 0.01 by two-way ANOVA with Bonferroni correction. (C) CDH5 protein expression at 24, 48, and 72 h time points after mRNA transfection were analyzed by western Blotting. The number above each gel lane represent the fold-change in intensity relative to control (TF only, 24 h).
Techniques Used: Functional Assay, In Vitro, Expressing, Transfection, Fluorescence, RNA Extraction, cDNA Synthesis, Western Blot, Control
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